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Bioline The PCR Company is an evolving international company, which develops, manufactures and markets a wide range of specialized bio-research reagents that simplify, accelerate and improve life sciences Research. Founded in 1992 and headquartered in London, UK., Bioline is an evolving international company, which develops, manufactures and markets a wide range of specialized bio-research reagents that simplify, accelerate and improve life sciences Research.

MWE offer microbiological Transport devices like Transwab or other innovative Solutions like  Virocult, Sigma-Swab (∑-Swab), Polywipe and Rapid Test Strips .

MWE has recently celebrated 60 years of Trading .Today MWE produces in excess of 100 million medical devices per annum.  The Transwab range is recognised as the market leading product of choice and the portfolio of products and prestigious clients continues to grow.

All clinical Transwab products comply with the CLSI standard M40-A (for the Quality Control of Microbiology Specimen Transport Devices).

Medical Wire’s clinical products are CE-marked as medical devices (up to Class IIa) or in vitro medical devices, manufactured to ISO 13485:2003. Where appropriate, they are registered with the United States FDA. All products are also manufactured to ISO 9001:2008.

Tri Reagent and derivates are the most famous products of MRC. Molecular Research Center, Inc., founded in 1989 by Piotr Chomczynski, develops and manufactures innovative products for the isolation and analysis of RNA and DNA. It is a corporation ” run by scientists for scientists” and they are continuously working on the development and improvement of molecular biology techniques.

Beginning with the single-step method for RNA Isolation they have worked to simplify nucleic acid isolation protocols while providing improved recovery and sample purity. This method has resulted in the most cited scientific publication in the last 25 years.

The patented RNA and DNA extraction techniques are recognized around the world as having the best performance, reliability and cost effectiveness.

TRI Reagent ® / TRI Reagent ® BD / TRI Reagent ® LS

TRI Reagent® is a patented reagent for the isolation of total RNA or for the simultaneous isolation of RNA, DNA and proteins. The reagent is an improved version of the popular single-step method for total RNA isolation (1,2,3). It is a monophase solution containing phenol and guanidine thiocyanate. TRI Reagent® provides a reliable, cost effective and efficient method of RNA isolation. TRI Reagent® allows for a comprehensive analysis of gene expression in a variety of samples of human, animal, plant, yeast, bacterial and viral origin. RNA isolation is complete in less than one hour, and DNA and protein isolations in less than three hours. Three versions of TRI Reagent® are available, each designed for optimal isolation efficiency from certain types of samples.

RNAzol® RT / RNAzol® BD

RNAzol® RT is the most effective reagent for isolation of total RNA and small RNA from samples of human, animal, plant, bacterial and viral origin. This patented reagent(1) provides higher yield and quality of isolated RNA than previous reagents based on the single-step method. RNAzol® RT isolates pure and undegraded RNA that is ready for RT-PCR without DNase treatment.(2)

  • No chloroform-induced phase separation is necessary to obtain pure RNA. Just add water to remove DNA, proteins, polysaccharides and other contaminants.
  • The isolation procedure can be completed in less than one hour and is performed at room temperature, including all centrifugation steps.
  • RNAzol® RT isolates total RNA, or large RNA and small RNA in separate fractions. The large RNA fraction contains rRNA and mRNA. The small RNA fraction contains tRNA, small RNA and microRNA down to 10 bases.
  • The isolated RNA is ready for RT-PCR, qRT-PCR, microarrays, poly A+ selection, northern blotting, RNase protection assay and other molecular biology applications.
  • Due to the removal of impurities, the RNA pellets are smaller and solubilize more easily than pellets obtained from previous single-step reagents.
  • In addition, RNAzol® RT allows for the simultaneous isolation of RNA and DNA

RNAzol® BD is the most effective reagent for isolation of total RNA from whole blood, plasma or serum of human or animal origin. This patented reagent(1) employs an improved single-step method to provide the highest yield and purity of isolated RNA. RNAzol® BD isolates pure and undegraded RNA that is ready for RT-PCR without DNase treatment (2).

  • RNAzol® RT isolates total RNA, or large RNA and small RNA in separate fractions. The large RNA fraction contains rRNA and mRNA. The small RNA fraction contains tRNA, small RNA and microRNA down to 10 bases.
  • RNAzol® BD allows for the isolation of RNA and DNA from the same blood sample.
  • The RNA isolation procedure can be completed in 1.5 hours and is performed at room temperature, including centrifugation steps.
  • Typical yields provide 8 – 22 μg total RNA per 1ml of human blood.
  • The isolated RNA is ready for RT-PCR, qRT-PCR, microarrays, poly A+ selection, northern blotting, RNase protection assay and other molecular biology applications.
  • Due to the removal of impurities, the RNA pellets are smaller and solubilize more easily.

DNAzol® / DNAzol® ES / DNAzol® BD

DNAzol® is a complete and ready to use reagent for the isolation of genomic or viral DNA from solid and liquid samples of human, animal and plant origin. The DNAzol procedure is based on the use of a novel guanidine – detergent lysing solution that hydrolyzes RNA and promotes the selective precipitation of DNA from the cell lysate (1, 2). This advanced DNA isolation method combines reliability and efficiency with the simplicity of a fast isolation protocol. DNAzol® is a non-toxic solution and the procedure does not require the use of phenols. DNA can be obtained from a large number of samples of small or large volume; 50 ml of DNAzol® is sufficient to process 50 samples, each with 25 – 50 mg of tissue. Genomic DNA isolation can be completed in 10 – 30 minutes with DNA recovery of 70 – 100%. Isolated DNA can be used for Southern analysis, dot blot hybridization, molecular cloning and polymerase chain reaction. There are 3 versions of DNAzol®, each designed for optimal isolation efficiency from various types of samples.

DNAzol ® Direct

DNAzol® Direct is a universal reagent for processing biological samples for direct PCR. No DNA isolation is required. The DNAzol Direct procedure is simple and fast. Lyse a sample in DNAzol Direct for 15 min, add an aliquot of the lysate to a PCR
mix, and perform amplification of a selected DNA fragment(s). DNAzol Direct simultaneously acts to release and denature DNA into a single-stranded form, hydrolyze RNA, and denature and partially hydrolyze proteins. The patent-pending DNAzol Direct composition and procedure are based on an alkaline solution containing polyethylene glycol and other additives. The combined effects of the alkaline pH and chaotropic properties of this reagent are sufficient to effectively inactivate PCR inhibitors including proteases and nucleases. This protocol eliminates columns, centrifugation steps and DNA precipitation. The standard DNAzol Direct procedure supports PCR amplification of DNA fragments up to 8 kb long.

DNAzol® Direct is designed to process a wide range of samples including animal, plant, fungi, yeast, bacterial, and viral samples. It has been used with blood, serum and plasma, saliva, buccal swabs, blood cards, hairs and feathers, leaves and seeds, and formalin-fixed tissues.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

xxpress qPCR Cycler

A 40 cycle qPCR is now possible in just 10 minutes.

With a thermal ramp rate of up to 10°C per second, the xxpress is the fastest, real-time thermal cycler in the world, delivering a 40 cycle qPCR in just 10 minutes. Whilst not all PCR chemistries can run ultra-fast protocols on the xxpress, there are a number of chemistries available that are ultra-fast compatible and will reliably achieve 40 cycles in 10 minutes.

Manual

You can order also in LabShop online www.labshop-online.com.

With ultra-low thermal mass and high conductivity, xxpress’ patented resistive heating system delivers its performance more efficiently and with greater accuracy than traditional based Solutions.

The Most Thermally Accurate qPCR Thermal Cycler in the World

Thermal Uniformity of ±0.3°C across all samples during cycling.

To enable a thermal uniformity of ±0.3°C across all the samples during cycling, the xxpress uses an array of highly accurate infrared sensors to determine the temperature of the samples. A patented control algorithm measures the temperature 100 times a second and adjusts the heating patterns 25 times a second. This ensures that thermal uniformity within the samples is better than ±0.8°C during ramping and ±0.3°C at static temperatures. This accuracy makes sure that all the samples across the plate experience the same thermal profile; creating consistent and comparable results from sample-to-sample, and from assay-to assay.

The Most Thermally Accurate qPCR Thermal Cycler in the World

Thermal Uniformity of ±0.3°C across all samples during cycling.

To enable a thermal uniformity of ±0.3°C across all the samples during cycling, the xxpress uses an array of highly accurate infrared sensors to determine the temperature of the samples. A patented control algorithm measures the temperature 100 times a second and adjusts the heating patterns 25 times a second. This ensures that thermal uniformity within the samples is better than ±0.8°C during ramping and ±0.3°C at static temperatures. This accuracy makes sure that all the samples across the plate experience the same thermal profile; creating consistent and comparable results from sample-to-sample, and from assay-to Assay.

Intuitive User Interface Designed by Biotechnologists for Biotechnologists

Built-in MIQE support and an intuitive Interface.

The xxpress system has an intuitive, touch screen user interface that has been designed to mimic the way a biotechnologist would develop their experiments. The software guides the user through the experiment set-up, selecting the chemistry utilised, PCR type, plate format and thermal profile. It even provides guidance based on the MIQE recommendations for experiment design.

Thermal profiles are provided for a variety of reagent kits, which can be simply adjusted if required. The xxpress displays amplification and melt curves as they develop so the user can see their data collecting in real-time. Results can be exported in two formats, RDML and ExcelTM, enabling simple analysis.

All previous protocols and run results are stored on the xxpress. These can be accessed from the home screen via the ‘History’ and ‘Analysis’ tabs respectively.

A Range of xxplate Volumes Ensures Optimum Reagent Use

MULTIPLE VOLUMES

xxplates are available in a range of sizes: 24, 54 and 96 wells. They have been designed around a standard form factor and are therefore interchangeable. The xxpress will recognise which plate size has been inserted and in which orientation, adjusting the layout of your results accordingly. Our range of plate sizes cover sample volumes from 1µl to 40µl, allowing optimal use of reagents and minimising the cost-per-test. Our 24 and 96 well plates are set on a standard pitch, compatible with auto pipetting Systems.

xxpress Provides Outstanding Return on Investment (ROI)

The xxpress system enables the optimal use of reagents and staff time. This significantly reduces the cost-per-test, making it Best in Class. With cycle times as little as 10 minutes, 5 experiments can be completed per hour, that’s more than 40 in a single shift.

The touch screen and built-in, windows embedded computer enables the xxpress to be completely self-contained. There’s no need for an external computer, monitor or keyboard.

Faster, more accurate temperature cycles mean that one xxpress can do the work of up to 6 standard qPCR thermal cyclers. With a foot print of only 300 mm wide by 590 mm deep, the xxpress saves bench space and minimises laboratory overheads.

Fast test cycles can lead to shorter development programs, products being released earlier and revenues being received sooner. The xxpress really is the perfect, qPCR thermal cycler for a modern laboratory.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

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