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Tri Reagent

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Molecular research center

Tri Reagent

Tri Reagent and derivates are the most famous products of MRC. Molecular Research Center, Inc., founded in 1989 by Piotr Chomczynski, develops and manufactures innovative products for the isolation and analysis of RNA and DNA. It is a corporation ” run by scientists for scientists” and they are continuously working on the development and improvement of molecular biology techniques.

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Beginning with the single-step method for RNA Isolation they have worked to simplify nucleic acid isolation protocols while providing improved recovery and sample purity. This method has resulted in the most cited scientific publication in the last 25 years.

The patented RNA and DNA extraction techniques are recognized around the world as having the best performance, reliability and cost effectiveness.

TRI Reagent ® / TRI Reagent ® BD / TRI Reagent ® LS

TRI Reagent® is a patented reagent for the isolation of total RNA or for the simultaneous isolation of RNA, DNA and proteins. The reagent is an improved version of the popular single-step method for total RNA isolation (1,2,3). It is a monophase solution containing phenol and guanidine thiocyanate. TRI Reagent® provides a reliable, cost effective and efficient method of RNA isolation. TRI Reagent® allows for a comprehensive analysis of gene expression in a variety of samples of human, animal, plant, yeast, bacterial and viral origin. RNA isolation is complete in less than one hour, and DNA and protein isolations in less than three hours. Three versions of TRI Reagent® are available, each designed for optimal isolation efficiency from certain types of samples.

RNAzol® RT / RNAzol® BD

RNAzol® RT is the most effective reagent for isolation of total RNA and small RNA from samples of human, animal, plant, bacterial and viral origin. This patented reagent(1) provides higher yield and quality of isolated RNA than previous reagents based on the single-step method. RNAzol® RT isolates pure and undegraded RNA that is ready for RT-PCR without DNase treatment.(2)

  • No chloroform-induced phase separation is necessary to obtain pure RNA. Just add water to remove DNA, proteins, polysaccharides and other contaminants.
  • The isolation procedure can be completed in less than one hour and is performed at room temperature, including all centrifugation steps.
  • RNAzol® RT isolates total RNA, or large RNA and small RNA in separate fractions. The large RNA fraction contains rRNA and mRNA. The small RNA fraction contains tRNA, small RNA and microRNA down to 10 bases.
  • The isolated RNA is ready for RT-PCR, qRT-PCR, microarrays, poly A+ selection, northern blotting, RNase protection assay and other molecular biology applications.
  • Due to the removal of impurities, the RNA pellets are smaller and solubilize more easily than pellets obtained from previous single-step reagents.
  • In addition, RNAzol® RT allows for the simultaneous isolation of RNA and DNA

RNAzol® BD is the most effective reagent for isolation of total RNA from whole blood, plasma or serum of human or animal origin. This patented reagent(1) employs an improved single-step method to provide the highest yield and purity of isolated RNA. RNAzol® BD isolates pure and undegraded RNA that is ready for RT-PCR without DNase treatment (2).

  • RNAzol® RT isolates total RNA, or large RNA and small RNA in separate fractions. The large RNA fraction contains rRNA and mRNA. The small RNA fraction contains tRNA, small RNA and microRNA down to 10 bases.
  • RNAzol® BD allows for the isolation of RNA and DNA from the same blood sample.
  • The RNA isolation procedure can be completed in 1.5 hours and is performed at room temperature, including centrifugation steps.
  • Typical yields provide 8 – 22 μg total RNA per 1ml of human blood.
  • The isolated RNA is ready for RT-PCR, qRT-PCR, microarrays, poly A+ selection, northern blotting, RNase protection assay and other molecular biology applications.
  • Due to the removal of impurities, the RNA pellets are smaller and solubilize more easily.

DNAzol® / DNAzol® ES / DNAzol® BD

DNAzol® is a complete and ready to use reagent for the isolation of genomic or viral DNA from solid and liquid samples of human, animal and plant origin. The DNAzol procedure is based on the use of a novel guanidine – detergent lysing solution that hydrolyzes RNA and promotes the selective precipitation of DNA from the cell lysate (1, 2). This advanced DNA isolation method combines reliability and efficiency with the simplicity of a fast isolation protocol. DNAzol® is a non-toxic solution and the procedure does not require the use of phenols. DNA can be obtained from a large number of samples of small or large volume; 50 ml of DNAzol® is sufficient to process 50 samples, each with 25 – 50 mg of tissue. Genomic DNA isolation can be completed in 10 – 30 minutes with DNA recovery of 70 – 100%. Isolated DNA can be used for Southern analysis, dot blot hybridization, molecular cloning and polymerase chain reaction. There are 3 versions of DNAzol®, each designed for optimal isolation efficiency from various types of samples.

DNAzol ® Direct

DNAzol® Direct is a universal reagent for processing biological samples for direct PCR. No DNA isolation is required. The DNAzol Direct procedure is simple and fast. Lyse a sample in DNAzol Direct for 15 min, add an aliquot of the lysate to a PCR
mix, and perform amplification of a selected DNA fragment(s). DNAzol Direct simultaneously acts to release and denature DNA into a single-stranded form, hydrolyze RNA, and denature and partially hydrolyze proteins. The patent-pending DNAzol Direct composition and procedure are based on an alkaline solution containing polyethylene glycol and other additives. The combined effects of the alkaline pH and chaotropic properties of this reagent are sufficient to effectively inactivate PCR inhibitors including proteases and nucleases. This protocol eliminates columns, centrifugation steps and DNA precipitation. The standard DNAzol Direct procedure supports PCR amplification of DNA fragments up to 8 kb long.

DNAzol® Direct is designed to process a wide range of samples including animal, plant, fungi, yeast, bacterial, and viral samples. It has been used with blood, serum and plasma, saliva, buccal swabs, blood cards, hairs and feathers, leaves and seeds, and formalin-fixed tissues.

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