SARS-CoV-2 ViroReal® Kit RT-LAMP
In-vitro diagnostic test for the detection of RNA from SARS-CoV-2 based on the one-step RT-LAMP technology.
- Amplification and detection of: ORF1ab from SARS-CoV-2
- Inhibited activity at room temperature
- PCR devices: runs on all common real-time PCR instruments and block PCR devices
- Available as: Kit (with reaction mix)
- The new SARS-CoV-2 variant from Great Britain (VOC-202012/01) is also recorded
SARS-CoV-2 ViroReal® Kit RT-LAMP
1
Benefits
- Used to screen patient samples of all age groups with and without suspected COVID-19 disease
- The RT-LAMP reaction takes between 30-53 minutes, depending on the (realtime) PCR device
- Suitable for both native (not extracted) and extracted test material
- Compatible with real-time PCR devices that detect fluorescence in the SYBR Green / FAM channel as well as with conventional block PCR devices
2
Description
- In-vitro diagnostic test for the detection of RNA from SARS-CoV-2
- Based on the one-step RT-LAMP technology
- Samples with an RNA concentration of approx. 20,000 copies per ml can be reliably detected with a sensitivity of 95% and a specificity of 99%
3
Test evaluation
- A real-time PCR device should be used to detect the RT-LAMP reaction
- After amplification, a visual check of the color change in the reaction tubes must be performed
- The drop of the pH value is due to the large amount of DNA produced
- The amplification takes 30 min at 63 ° C.
- The analysis of the melting curve takes between 5 and 13 minutes
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