LabQ RealTime PCR Kit SYBR Universal
- Allround-qPCR Mix for SYBR®Green-based detection
- Optimized for a broad range of applications
- Reduces the risk of contamination from PCR products
- Suitable for all PCR machines
- 2000 rxn á 20µl (4x5ml)
LabQ RealTime PCR Kit SYBR Universal
Includes
- HotStart feature for maximum control over the reaction start (e.g. in automated applicatons).
- dUTP/dTTP blend to enable UNG digestion (reducing the risk of contamination from PCR products).
- Universal ROX concentration suitable for all PCR machines.
- Additional normalization option for Bio-Rad cyclers.
- Improved perfomance in the presence of PCR inhibitor.
Additional materials required
- Nuclease-free PCR tubes or plates and suitable sealing options.
- Real-time PCR cycler.
- PCR primer.
- Template DNA and control DNA standards.
- Filter pipette tips.
- Sterile, nuclease-free, DNA-free tubes for preparing the reaction mix.
Reaction Setup
Before starting the reaction setup, thaw LabQ RealTime PCR Kit SYBR Universal and mix thoroughly but gently to ensure even distribution of components.
Dilute your standard DNA and experimental samples with nuclease-free water to the desired concentrations and add them to theier designated wells in the multi-well plate.
For negative control, add nuclease-free water.
Keep the plate on ice until further use.
| Component | Volume | Final Concentration |
| LabQ RealTime PCR Kit SYBR Universal | 10 µl | 1x |
| Template DNA | < 10 ng for low complexity templates | |
| X µl | >1 for high complexity templates | |
| Max. 2µl of reverse transcription reaction | ||
| Forward primer (10µM) | 0.4 µl | 0.05 – 0.09 µM each |
| Reverse primer (10µM) | 0.4 µl | 0.05 – 0.9 µM each |
| Nuclease-free dH2O | To 20 µl |
Recommended qPCR Protocol
| Set | Cycles | °C | Time |
| Initial Denaturation | 1 | 95°C | 5 minutes |
| Amplification | 40 | 95°C
55-60° 72°C |
10 seconds
15 seconds / 10 seconds 20 seconds |
Add an addtional melting curve step if required. A melting curve is recommended to ensure specific amplification and to detect possible primer oligomers.
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LabConsulting is a Viennese company that cooperates with over 600 manufacturers and offers a selection of more than 180,000 products and various laboratory solutions.
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