SensiFAST™ cDNA Synthesis Kit

SensiFAST™ cDNA Synthesis Kit, 250 reactions
  • Unique reverse transcriptase and new 5x TransAmp™ buffer generates consistent first-strand cDNA
  • Ideal for dilute and low-copy samples
  • Real-Time RT-PCR analysis from as little as 1pg of starting total RNA
  • Complete 5' to 3' RNA sequence representation
  • Optimized protocol produces cDNA in 15 minutes
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SensiFAST™ cDNA Synthesis Kit

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Product Highlights

  • Efficient – high target affinity, coupled with a novel TransAmp™ buffer system for improved yield of full length cDNA.
  • Unbiased – optimized mix of random hexamers and anchored oligo dT primers for complete 5’ to 3’ RNA sequence representation.
  • Sensitive – lower Ct values from a broad range of input cDNA concentrations enabling accurate detection of dilute (1 pg of input RNA) and even very low copy targets.
  • Robust – reliable reverse transcription under challenging conditions, including complex templates and in the presence of inhibitors.
  • Fast – high-yield reverse transcription from a very broad of targets in as little as 5 minutes.
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Applications

  • Gene expression analysis.
  • Tissue biopsy analysis.
  • miRNA profiling / quantification.
  • RNA target detection.
  • Pathogen detection.

The SensiFAST cDNA Synthesis Kit has been developed to generate real-time ready first strand cDNA from total RNA templates.
The kit contains a unique TransAmp™ buffer system to deliever the highest quality cDNA.
A blend of random and oligo(dT) primers provide robust, consistent and unbiased first-strand synthesis over a broad range of RNA template concentrations, making SensiFast cDNA Synthesis Kit the ideal partner for SensiFAST Probe and SYBR real-time PCR kits.

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